Web30-2004 ™. F-12K Medium (Kaighn's Modification of Ham's F-12 Medium) contains 2 mM L-glutamine and 1500 mg/L sodium bicarbonate. It has applications for cell culture, cell … WebOct 29, 2024 · Human alveolar epithelial cells (A549, ATCC) were maintained in F-12K medium (Gibco) supplemented with 10% FBS (Atlanta Biologicals), 1% antibiotic/antimycotic solution (Gibco), and 10 mg/mL gentamicin (Gibco). Both of the cell lines were kept at 37 °C, 5% CO 2, until infection.
Formulation for F-12K Medium 30-2004 - ATCC
WebA549 can be ordered from ATCC as a frozen ampoule. This is an adherent cell line. Name: A549—Human Lung Carcinoma Epithelial Cells ATCC #: CCL-185 Materials List 1. F-12K Medium (ATCC, Cat# 30-2004) 2. Characterized Fetal Bovine Serum (HyClone, Cat# SH30071) 3. T25, T75, T225 tissue culture flasks 4. http://genome.cse.ucsc.edu/ENCODE/protocols/cell/human/BE2-C_Stam_protocol.pdf fashion kids las arenas
Amaxa® Cell Line Nucleofector® Kit T - Lonza
WebF 12k Culture Medium, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more ... CCL-61) cells were maintained in F-12k culture medium (Kaighn's Modification of Ham's F-12 Medium, ATCC, 30-2004) with 10% Fetal Bovine Serum … WebSep 19, 2014 · A549 cells (ATCC ® CCL-185 ™) were maintained in F-12K Medium (ATCC ® Catalog No. 30-2004) with 10% FBS and kept in a 37°C incubator with a 5% CO 2 air atmosphere. Cells were transfected with 0.2 μl/well Dharmafect I (Thermofisher Scientific) as per the manufacturers' protocol. TGF-β was added at the time of transfection. Cells were ... WebATCC F-12K Medium (Kaighn's Modification of Ham's F-12 Medium), 500mL. Supplier: ATCC 302004. It is modified to contain 2 mM L-glutamine and 1500 mg/L sodium … fashion kids ribeirão preto