Flp-in 293 cells

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August undefined, 2024

WebJan 6, 2024 · I am trying to generate stable cell line by using Flp-In 293 T-REx cells. I use 9:1 (w/w) ratio of pOG44:pcDNA-GOI and lipofectamine as a transfection reagent. After 48 hours transfection, I start ... WebArticle Snippet: Flp-In T-Rex Human Embryonic Kidney 293 (HEK293) cells were purchased from ThermoFisher Scientific, while the parental HEK293 cell line was obtained from ATCC (Manassas, VA, USA). Techniques: High Throughput Screening Assay, Promoter Assay, Derivative Assay

Hek293 Flp In T Rex 293 Thermo Fisher Bioz

WebFlp-In ™ T-REx -293 cell line. Complete Medium [Antibiotic] Freezing Medium D-MEM (high glucose) 10% FBS 2 mM L-glutamine 1% Pen-Strep (optional) 100 μg/mL Zeocin™ 15 μg/mL blasticidin 90% complete medium 10% DMSO Important Guidelines Consider the … WebJul 4, 2024 · In the present study, we employed Flp-In-293 cells with the Flp-In™ system to establish cells expressing WT or non-synonymous SNP variants of human ABCC4 (Figure 1 and Table 1). Flp-In-293 cells were transfected with the ABCC4 cDNA, which integrated … dark wood essential oil diffuser

Affinity Purification of Soluble and Membrane-Bound Protein

WebE , Flp-In 293 background cells or cells expressing NUBP2 were either directly harvested or after treatment with 1% formaldehyde. Anti-HA immunoprecipitation were performed. WCEs and anti-HA … WebAug 20, 2024 · The Flp-In system (Thermo Fisher Scientific, Darmstadt, Germany) uses the Flp recombinase to generate isogenic cell lines, in which the gene of interest integrates at a single well-defined locus ... WebThe Flp-In™ T‑REx™-293 cell line is derived from 293 human embryonic kidney cells (Graham et al., 1977). The 293 parental cell line was obtained from the American Type Culture Collection (ATCC®). For more information about the 293 parental cell line, see … dark wooden table background

Invitrogen™ Flp-In™ T-REx™ 293 Cell Line - fishersci.com

WebSep 12, 2024 · The Flp-In™ System (Invitrogen) was used to generate a cell line expressing Halo-HDAC1 under the control of the CMVd2 promoter in Flp-In™-293 host cells essentially as described previously 55 ... WebCell Line Source Amount Catalog no. Flp-In™-293 Human embryonic kidney 1 × 107 cells, frozen R750–07 Flp-In™-CV-1 African Green Monkey kidney 1 × 107 cells, frozen R752–07 Flp-In™-CHO Chinese Hamster ovary 1 × 107 cells, frozen R758–07 Flp-In™-BHK … dark wood extending tableWebMay 1, 2008 · We expressed a total of seven non-synonymous SNP (single nucleotide polymorphism) variants in Flp-In-293 cells by using the Flp (flippase) recombinase system. Of these, ABCG2 F208S and S441N variants were found to be expressed at markedly low levels, whereas their mRNA levels were equal to those of the other SNP variants and … dark wood fence stain

"WebFlp-In™ 293 cell line is designed for rapid generation of stable cell lines that ensure high level expression of your protein of interest from a Flp-In™ expression vector. These cells contain a single stably integrated FRT site at a transcriptionally active … Targeted integration of a Flp-In™ expression vector ensures high-level … TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes " - Flp-in 293 cells

Flp-in 293 cells

WebJun 6, 2015 · I am trying to generate stable cell line by using Flp-In 293 T-REx cells. I use 9:1 (w/w) ratio of pOG44:pcDNA-GOI and lipofectamine as a transfection reagent. After 48 hours transfection, I start ... WebApr 1, 2016 · Symbols and abbreviations are as described in Fig. 1. (E) Flp-In-293 cells were transiently transfected with the indicated GPR37 constructs for 24 h, and the anti-Myc-antibody-labelled full-length receptors were analysed by confocal microscopy as …

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WebI am trying to generate stable cell line by using Flp-In 293 T-REx cells. I use 9:1 (w/w) ratio of pOG44:pcDNA-GOI and lipofectamine as a transfection reagent. After 48 hours transfection, I start ... WebNov 8, 2016 · The FH-AKAP95 and FH-AKAP95 (101–692) cell lines were made by co-transfecting Flp-In-293 cells (f293 cells, Invitrogen) with pOG44 plasmid and either an FH-AKAP95-pcDNA5 or an FH-AKAP95 (101 ...

WebThe cell line was initiated by the transformation and culturing of normal HEK cells with sheared adenovirus 5 DNA. A portion of human adenovirus 5, nucleotides (nts) 1-4344, is integrated into chromosome 19 of the HEK cells. Human adenovirus 5 causes a range of respiratory illnesses. HEK 293 cell lines are commonly used in cancer research. WebIn hES cells where LIN28 is expressed at high levels, we performed CLIP-seq with an antibody that specifically recognizes the endogenous protein (Figures 1A and S1A).To model the reactivation of LIN28 expression observed in many cancer cells, we generated a stable Flp-In HEK293 cell line that constitutively expresses a C-terminal V5-tagged …

WebApr 6, 2024 · As most GBA1 mutations lead to a misfolded enzyme that is rapidly degraded via ER-associated degradation by the proteasome 11,12, we generated stable Flp-In™T-REx™-HEK 293 cell lines ... WebRNA-seq of the NE cells homozygous for W1282X showed that CFTR transcript level was reduced to 1.7% of WT (p-value: 4.6e-3). Negligible truncated CFTR protein was generated by Flp-In 293 cells stably expressing the W1282X EMG even though CFTR transcript was well above levels observed in the parents and proband.

WebFeb 4, 2024 · inducible cell lines to express either hnRNPA1 wild type or D262V mutant epitope-tagged cDNAs. These cells were generated using FLP-recombinase mediated integration in 293 cells, while expressing endogenous wild type hnRNPA1. This experimental set up was initially designed to mimic ALS patients carrying a heterozygous …

WebFlp-In 293 cell line is designed for rapid generation of stable cell lines that ensure high level expression of your protein of interest from a Flp-In expression vector. These cells contain a single stably integrated FRT site at a trans dark wood file cabinetWebThe protein expression level of the Q141K variant increased about two-fold when Flp-In-293 cells were treated with MG132. In contrast, the protein level of ABCG2 WT was little affected by the same treatment. After treatment with bafilomycin A1, the protein levels of ABCG2 WT and Q141K increased 5- and 2-fold in Flp-In-293 cells, respectively. dark wood filing cabinets ukWebT-REx™ Cell Lines For your convenience, Invitrogen has available three mammalian cells lines that stably express the Tet repressor. T-REx™-293 cells and T-REx™-HeLa cells express the Tet repressor from pcDNA™6/TR and should be maintained in medium containing blasticidin. T-REx™-U2OS cells express bis-hydroxyethoxypropyl dimethiconeWebFlp-In 293 cell line is designed for rapid generation of stable cell lines that ensure high level expression of your protein of interest from a Flp-In expression vector. Targeted integration of a Flp-In expression vector ensures high-level expression of your gene of interest. Flp-In-293 cell line was created by transfecting the parent cell ... bishy boatsWebOct 15, 2024 · To test the integration activity of the Flp-TAL recombinases, we transfected HEK-293 cells with the vectors that express FV61-TAL, FV63-TAL, or FV71-TAL and the corresponding pTarget variant. 48 h ... bishy barney boats bis hydrogenated tallow alkyl dimethylWebFlp-In 293 cell line is designed for rapid generation of stable cell lines that ensure high level expression of your protein of interest from a Flp-In expression vector. Targeted integration of a Flp-In expression vector ensures high-level expression of your gene of interest. Flp-In … dark wood fence panels